Along with over a decade rich experience in providing excellent custom scaffold protein library construction services, now Creative Biolabs offers the unique Fynomers library construction service for our customers all over the world. We are pleased to use our proprietary Hi-Affi™ platform to generate the scaffold libraries with 100% precise mutation and over 1010 diversity.
Introduction of Fynomers
In recent years, the generation of binding molecules based on scaffold proteins have the huge potential to replace antibodies in the biological research and applications. Fyn is a 59 kDa member of the Src family of tyrosine kinases. In general, the Fyn SH3 can be composed with two antiparallel β-sheets and also contains two flexible loops which are RT loop and n-Src loop. These two loops play the important role in the protein interactions. Fynomers are small binding proteins (7 kDa) derived from the human SH3 domain of Fyn kinase which can be engineered to yield specific and high-affinity binding domains to target the specific proteins.
Fig.1 The Fyn SH3 protein structure and library cloning strategy. (Grabulovski, D.; et al. 2007)
Construction of Fynomers Library
As for the Fynomers library construction, the gene encoding the Fyn SH3 domain can be amplified from the human cDNA library. With a series of researches, now Creative Biolabs has got the sequence of Fyn SH3 domain and the position sites of these two flexible loops. After the polymerase chain reaction with the corresponding primers, the resulting DNA fragment can then be inserted into the vector to form the Tyn SH3 WT. The most important stage of Fynomers library construction is the randomization of two flexible loops of the SH3 fold. In this case, the Tyn SH3 WT serves as the template and the PCRs are performed with the partially degenerate primers. And then, the randomized insert can be double-digested and cloned into the vector. After the electrical E. coli transformation, we can finally obtain the Fynomers library.
Attractive Features of Fynomers
Creative Biolabs is one of the well-recognized experts who are professional in applying advanced phage display technologies for a broad range of scaffold protein research. Based on our extensive experience and professional experts, we are therefore confident in offering the best and most suitable service for our customers. If you are interested in our Fynomers library construction service, please do not hesitate to inquire us for more details.
Reference
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.